Electrospun Poly-Lactic Acid Nanofibers as a scaffold for Regenerative Endodontics purposes: Fabrication and Characterization

Poly (lactic acid) (PLA), which is a polyester, undergoes hydrolytic degradation into lactic acid, a natural metabolic byproduct, making it well-suited for medical applications. PLA nanofibers have expanded the potential applications of PLA scaffolds, particularly in regenerative medicine and drug delivery. These nanofibrous scaffolds offer several advantageous features, including a high surface area, the ability to mimic the architecture of the native extracellular matrix, and tunable mechanical properties (factors that are crucial when designing scaffolds for specific organ systems). This study deals with the synthesis, characterization, and evaluation of PLA nanofibrous scaffold. For the fabrication. Polylactic acid (PLA) polymer solution at a concentration of 25 wt% was processed into Fibres. Characterization used in the current study included scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), degradation tests. The antimicrobial properties were tested against Porphyromonas gingivalis (Pg) and Enterococcus faecalis (Ef). SEM imaging showed Fibres with submicron diameters, and FTIR spectra of nanofibers showed that PLA was evident by the presence of the characteristic peaks. Antimicrobial activity was confirmed via agar diffusion assays, showing evidence of antimicrobial activity against the tested bacteria. These findings suggest that PLA scaffolds could serve as a biologically safe system for further use in regenerative endodontics.