Green Synthesis, Characterization and Antimicrobial Activities of Silver Nanoparticles against some Phypathogenic Bacteria

Green chemistry methods are now an intriguing field of study in agriculture, particularly in pest management. For this reason, novel approaches for the more efficient manufacturing of nanoparticles with improved biological characteristics have been developed. Because nanoparticle production is faster, this method is more eco-friendly and less toxic than old methods. The biosynthesis of silver nanoparticles (AgNPs) using Leucaena leucocephala , Mentha aquatica , and Zingiber officinale extracts and their antibacterial activity against Pectobacterium cartovorum , Agrobacterium tumefaciens , and Xanthomonas axonopodis . The results showed that spectroscopic and microscopic methods, such as UV-Vis spectroscopy, revealed absorption peaks for Ll-AgNPs at 415 nm, 420 nm for Ma-AgNPs, and 430 nm for Zo-AgNPs, indicating the silver nature of the prepared colloidal samples. The TEM images revealed the quasi-spherical morphology of NPs with an average size of 12.51, 10.63, and 10.26 nm for Ll-AgNPs, Ma-AgNPs, and Zo-AgNPs. The X-ray diffraction (XRD) pattern revealed a face-centered cubic (FCC) structure with crystallite. While distinctive peaks in an investigation using Fourier transform infrared (FT-IR) spectroscopy showed that several biomolecules were attached to AgNPs, antibacterial activity was evaluated by an inhibitory zone test, which showed high efficiency against P. cartovorum , A. tumefaciens , and X. axonopodis , with an antibacterial function comparable to L. leucocephala , M. aquatica , and Z. officinale extract. The green production of silver nanoparticles has the potential to be a useful tool in pest management strategies against phytopathogenic bacteria.