Spectral Fingerprinting of Tencha Processing: Optimising the Detection of Total Free Amino Acid Content in Processing Lines by Hyperspectral Analysis

The quality and flavor of tea leaves are significantly influenced by chemical composition, with the content of free amino acids serving as a key indicator for assessing the quality of Tencha. Accurately and quickly measuring free amino acids during tea processing is crucial for monitoring and optimizing production processes. However, traditional chemical analysis methods are often time-consuming and costly, limiting their application in real-time quality control. Hyperspectral imaging (HSI) has shown significant effectiveness as a component detection tool in various agricultural applications. This study employs VNIR-HSI combined with machine learning algorithms to develop a model for visualizing the total free amino acid content in Tencha samples that have undergone different processing steps on the production line. Four pretreating methods were employed to preprocess the spectra, and partial least squares regression (PLSR) and least squares support vector machine regression (LS-SVR) models were established from the perspectives of individual processes and the entire process. Combining competitive adaptive reweighted sampling (CARS) and variable iterative space shrinkage approach (VISSA) methods for characteristic band selection, specific bands were chosen to predict the amino acid content. By comparing modeling evaluation indicators for each model, the optimal model was identified: the overall model CT+CARS+PLSR, with predictive indicators Rc² = 0.9885, Rp² = 0.9566, RMSEC = 0.0956, RMSEP = 0.1749, RPD = 4.8021, enabling the visualization of total free amino acid content in processed Tencha leaves. Here, we establish a benchmark for machine learning-based HSI, integrating this technology into the tea processing workflow to provide a real-time decision support tool for quality control, offering a novel method for the rapid and accurate prediction of free amino acids during tea processing. This achievement not only provides a scientific basis for the tea processing sector but also opens new avenues for the application of hyperspectral imaging technology in food science.