Coatomer complex I is required for the transport of SARS-CoV-2 progeny virions from the endoplasmic reticulum-Golgi intermediate compartment
SARS-CoV-2 virions are synthesized within the ERGIC and are transported to the cell surface via vesicular transport for release. However, the precise mechanisms remain unclear. Through various electron microscopic techniques, we identified the presence of COPI on virion-transporting vesicles. Alterations in the distribution of COPI and ERGIC in SARS-CoV-2 infected cells are evident, suggesting their involvement in virus replication. When COPB2, a component of COPI, is depleted, progeny virions become trapped within the ERGIC, leading to a reduction in the efficiency of virion release. These findings highlight COPI's crucial role in mediating SARS-CoV-2 vesicular transport from the ERGIC and suggest it as a potential antiviral target.
Authors
- Yoko Fujita (ORCID: https://orcid.org/0000-0002-2411-5473)
- Mayumi Wakazaki
- Takeshi Noda (ORCID: https://orcid.org/0000-0002-0658-4663)
- Yukiko Muramoto (ORCID: https://orcid.org/0000-0002-8706-3113)
- Ai Hirabayashi (ORCID: https://orcid.org/0000-0002-6209-5960)
- Chiho Onishi
- Koji Yamauchi
- Mayuko Sato (ORCID: https://orcid.org/0000-0002-9876-5612)
- Kiminori Toyooka (ORCID: https://orcid.org/0000-0002-6414-5191)
- Toru Takenaga (ORCID: https://orcid.org/0000-0002-1277-4156)
- Norimichi Nomura (ORCID: https://orcid.org/0000-0002-6330-2239)
- Yugo Tsunoda (ORCID: https://orcid.org/0000-0001-6415-4859)
- Masahiro Nakano (ORCID: https://orcid.org/0000-0003-4247-9008)
Institutions
- Kyoto University (JP)
- Saitama Prefecture (JP)
- Japan Science and Technology Agency (JP)
- RIKEN Center for Sustainable Resource Science (JP)
- Material Sciences (United States) (US)
Publication Details
- Journal
- mBio
- Published
- 2024-11-29
- DOI
- https://doi.org/10.1128/mbio.03331-24
- Citations
- 5
- Primary Topic
- SARS-CoV-2 and COVID-19 Research
- Type
- article
- Field-Weighted Citation Impact
- 1.95
Funders
- Takeda Science Foundation
- Japan Agency for Medical Research and Development
- Ministry of Education, Culture, Sports, Science and Technology
- University of Tokyo
- Japan Society for the Promotion of Science
- RIKEN
- Core Research for Evolutional Science and Technology