A novel synthetic synovial fluid model for investigating biofilm formation and antibiotic susceptibility in prosthetic joint infections
Infections after joint replacement are rare but can lead to severe complications as they are difficult to treat due to the ability of pathogens to form surface-attached biofilms on the prosthesis as well as biofilm aggregates in the tissue and synovial fluid. This biofilm phenotype, combined with the microenvironment at the infection site, substantially increases antimicrobial tolerance. Conventional <i>in vitro</i> models typically use standard growth media, which do not consider the microenvironment at the site of infection. By replacing these standard growth media with an <i>in vivo</i>-like medium, such as the synthetic synovial fluid medium, we hope to expand our knowledge on the aggregation of pathogens in the context of PJI. In addition, we believe that inclusion of <i>in vivo</i>-like media in antimicrobial susceptibility testing might be able to more accurately predict the <i>in vivo</i> susceptibility, which could ultimately result in a better clinical outcome after antimicrobial treatment.
Authors
- Bram Lories (ORCID: https://orcid.org/0000-0001-5767-7440)
- Frits van Charante (ORCID: https://orcid.org/0000-0002-5255-2048)
- Amber De Bleeckere (ORCID: https://orcid.org/0000-0003-1667-1901)
- Hans P. L. Steenackers (ORCID: https://orcid.org/0000-0001-5021-2069)
- Fabien Lamret (ORCID: https://orcid.org/0000-0002-4012-4214)
- Stien Vandendriessche (ORCID: https://orcid.org/0000-0002-3501-0140)
- Fany Reffuveille (ORCID: https://orcid.org/0000-0002-0753-3761)
- Martine De Cock (ORCID: https://orcid.org/0000-0001-7917-0771)
- T. Debord
- Matthias Verstraete (ORCID: https://orcid.org/0000-0002-4708-4235)
- Jerina Boelens (ORCID: https://orcid.org/0000-0003-2282-8194)
- Tom Coenye (ORCID: https://orcid.org/0000-0002-6407-0601)
Institutions
- Ghent University Hospital (BE)
- Ghent University (BE)
- European Society of Clinical Microbiology and Infectious Diseases (CH)
- Ecologie microbienne (FR)
- Biomatériaux et Inflammation en Site Osseux (FR)
- Université de Reims Champagne-Ardenne (FR)
- KU Leuven (BE)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2024-11-29
- DOI
- https://doi.org/10.1128/spectrum.01980-24
- Citations
- 8
- Primary Topic
- Orthopedic Infections and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 4.58
Funders
- European Society of Clinical Microbiology and Infectious Diseases
- Aalborg Universitet
- Fonds Wetenschappelijk Onderzoek
- Vlaamse regering
- Universitätsklinikum Hamburg-Eppendorf