Cryo-EM structure of a photosystem I variant containing an unusual plastoquinone derivative in its electron transfer chain

Photosystem I (PS I) is a light-driven oxidoreductase responsible for converting photons into chemical bond energy. Its application for renewable energy was revolutionized by the creation of the MenB deletion (Δ<i>menB</i>) variant in the cyanobacterium <i>Synechocystis</i> sp. PCC 6803, in which phylloquinone is replaced by plastoquinone-9 with a low binding affinity. This permits its exchange with exogenous quinones covalently coupled to dihydrogen catalysts that bind with high affinity, thereby converting PS I into a stable solar fuel catalyst. Here, we reveal the 2.03-Å-resolution cryo-EM structure of a recent MenB variant of PS I. The quinones and their binding environment are analyzed in the context of previous biophysical data, thereby enabling a protocol to solve future PS I hybrids and constructs from this genetically tractable cyanobacterium.

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Publication Details

Journal
Science Advances
Published
2024-11-29
DOI
https://doi.org/10.1126/sciadv.adp4937
Citations
2
Primary Topic
Photosynthetic Processes and Mechanisms
Type
article
Field-Weighted Citation Impact
0.47

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article

Cryo-EM structure of a photosystem I variant containing an unusual plastoquinone derivative in its electron transfer chain

Vasily Kurashov, K. V. Lakshmi, David F. Iwig, David J. Vinyard et al.
2 citations
Science Advances
Photosynthetic Processes and Mechanisms
0.47
article

Cryo-EM structure of a photosystem I variant containing an unusual plastoquinone derivative in its electron transfer chain

Vasily Kurashov, K. V. Lakshmi, David F. Iwig, David J. Vinyard, John H. Golbeck, Gary W. Brudvig, Brandon P. Russell, Christopher J. Gisriel
article en
2 citations

Abstract

Photosystem I (PS I) is a light-driven oxidoreductase responsible for converting photons into chemical bond energy. Its application for renewable energy was revolutionized by the creation of the MenB deletion (Δ<i>menB</i>) variant in the cyanobacterium <i>Synechocystis</i> sp. PCC 6803, in which phylloquinone is replaced by plastoquinone-9 with a low binding affinity. This permits its exchange with exogenous quinones covalently coupled to dihydrogen catalysts that bind with high affinity, thereby converting PS I into a stable solar fuel catalyst. Here, we reveal the 2.03-Å-resolution cryo-EM structure of a recent MenB variant of PS I. The quinones and their binding environment are analyzed in the context of previous biophysical data, thereby enabling a protocol to solve future PS I hybrids and constructs from this genetically tractable cyanobacterium.

Science AdvancesVol. 10(48)
Louisiana State University (US), Pennsylvania State University (US), Howard Hughes Medical Institute (US), University of Wisconsin–Madison (US), Rensselaer Polytechnic Institute (US), Yale University (US)
U.S. Department of Energy, Yale University, National Institute of General Medical Sciences, Basic Energy Sciences
Affordable and clean energy
Openalex Percentile: Top 34%
Photosynthetic Processes and Mechanisms
0.47
DOIOpen Access PDFOpenAlex
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Cryo-EM structure of a photosystem I variant containing an unusual plastoquinone derivative in its electron transfer chain — Vasily Kurashov, K. V. Lakshmi, et al. · Science Advances (2024) | TGRS Research Map | TGRS