Light-sheet dynamic scattering imaging of microscopic blood flow

Microcirculation imaging is crucial in understanding the function and health of various tissues and organs. However, conventional imaging methods suffer from fluorescence label dependency, lack of depth resolution, and quantification inaccuracy. Here, we report a light-sheet dynamic light-scattering imaging (LSH-DSI) system to overcome these shortcomings. LSH-DSI utilizes selected plane illumination for an optical sectioning, while a time-frequency analysis method retrieves blood flow velocity estimates from dynamic changes in the detected light intensity. We have performed imaging experiments with zebrafish embryos to obtain angiographs from the trunk and head regions. The results show that LSH-DSI can capture label-free tomographic images of microvasculature and three-dimensional quantitative maps of local blood flow velocities.