Inside-out submitochondrial particles affect the mitochondrial permeability transition pore opening under conditions of mitochondrial dysfunction
The inside-out submitochondrial particles (IO-SMPs) showed a strong protective effect against mitochondrial permeability transition pore (mPTP) opening in mitochondria isolated from swine hearts 3 h after explantation. The latter condition was used to emulate situation of mitochondrial damage. We identified that the protective effect of IO-SMPs cannot be attributed to a functional modulation of the enzymatic complexes involved in mPTP formation. Indeed, oxidative phosphorylation and F<sub>1</sub>F<sub>O</sub>-ATPase activity were not affected. Conversely, mPTP desensitization might be caused by structural modification. IO-SMP incorporation into the mitochondria can modulate the membrane-bound enzyme complexes' functionality, inducing F<sub>1</sub>F<sub>O</sub>-ATPase to be unable to carry out the conformational changes useful for mPTP opening. Thus, the data are a valid starting point for IO-SMP application in the treatment of impaired cardiovascular conditions supported by mPTP opening.
Authors
- Cristina Algieri (ORCID: https://orcid.org/0000-0002-1742-9378)
- Fabiana Trombetti (ORCID: https://orcid.org/0000-0003-1611-8727)
- Silvia Granata (ORCID: https://orcid.org/0000-0001-6512-1522)
- Maria Laura Bacci (ORCID: https://orcid.org/0000-0001-6702-5868)
- Salvatore Nesci (ORCID: https://orcid.org/0000-0001-8569-7158)
- Micaela Fabbri
- Patrycja Anna Glogowski (ORCID: https://orcid.org/0009-0004-2650-7034)
- Antonia Cugliari
Institutions
- Istituto Neurologico Mediterraneo (IT)
- University of Bologna (IT)
Publication Details
- Journal
- Biochimica et Biophysica Acta (BBA) - Bioenergetics
- Published
- 2024-11-28
- DOI
- https://doi.org/10.1016/j.bbabio.2024.149528
- Primary Topic
- Mitochondrial Function and Pathology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Università di Bologna