Proliferative Effects of <i>Curcuma zedoaria</i> Extract on Ovarian Tumor Cells

Background Curcuma zedoaria is a common traditional herbal medicine that contains mainly curcumin and volatile oils with antioxidant, anti-inflammatory, and antitumor properties. Objectives This study aimed to investigate the effects of C. zedoaria extract on the biological behavior of ovarian cancer (OC) cells. Materials and Methods Active compounds were extracted from C. zedoaria , and the content of different active compounds was quantified. Their scavenging abilities against DPPH and -OH free radicals were analyzed, and the minimum inhibitory concentrations (MICs) against different bacterial strains were determined. The human OC cell line SK-OV-3 was divided into four groups and cultured in media containing C. zedoaria extract at concentrations of 0.0, 0.3, 0.6, and 1.2 g/L. The proliferation, inhibition, migration, and apoptosis of primary OC cells were then assessed using the methyl tetrazolium (MTT) assay, the scratch-healing assay, and flow cytometry. Tumor cell invasion and clonogenicity were also assessed. Finally, the status of MEK/ERK signaling in the cells was examined by protein imprinting. Results C. zedoaria extract contained 0.18% curcumin, 0.04% demethoxycurcumin, and 0.02% bisdemethoxycurcumin. When the concentration of C. zedoaria extract was ≥10 mg/mL, it exhibited a scavenging rate of over 85% for DPPH and -OH free radicals. In addition, C. zedoaria extract effectively inhibited the growth of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus , and Staphylococcus epidermidis , with lower MICs observed for gram-positive bacteria. MTT cell proliferation and scratch assay results showed significant inhibition of SK-OV-3 cell proliferation and migration by C. zedoaria extract. Flow cytometry results indicated that the extract strongly promoted apoptosis in SK-OV-3 cells. Compared with the control group, the invasive and clonogenic abilities of SK-OV-3 cells decreased with increasing concentration of the extract. Protein imprinting results showed that treatment with C. zedoaria extract led to a concentration-dependent decrease in p-MEK1/2 and p-ERK1/2 protein levels in SK-OV-3 cells. Conclusion C. zedoaria extract possesses potent antioxidant, antibacterial, and antitumor properties. In addition, C. zedoaria extract can suppress the activation of MEK/ERK signaling, thereby inhibiting the proliferation and migration of SK-OV-3 cells, inducing apoptosis, and reducing the invasive and clonogenic potential of SK-OV-3 cells. It provides a theoretical basis for the discovery of novel drugs using C. zedoaria extract and its clinical applications in the treatment of OC.