Comparison between the size of PRF clot obtained through horizontal centrifugation vs. fixed-angle centrifugation

Introduction: Over the years, centrifugation systems have undergone significant transformations, adapting in shape to facilitate their application in dental clinics and advancing in operational technology. While centrifuge designs may vary in numerous ways, one critical distinction between the centrifuge is the angle at which centrifugation takes place. Aim of the Study: The objective of this experimental study is to assess and measure the volume of the end product, specifically the PRF clot obtained using horizontal centrifugation and fixed-angle centrifugation to ascertain which method produces a larger quantity of PRF clot. Material and Methods: The study was conducted on a cohort of 6 male and female patients. Blood was drawn from each patient into two 10ml glass tubes without anticoagulants or additives, destined for centrifugation in the two centrifugation systems. The tubes were split into two sets of six tubes each, labeled with the letters H (for tubes to be centrifuged horizontally) and L (for tubes to be centrifuged at a fixed angle), along with numbers from 1 to 6 for each tube pair corresponding to a patient. Results and Discussions: It has been proven that horizontal centrifugation produces a bigger PRF clot. Considering the insights gained from this study alongside the ANOVA test results, we conclude that horizontal centrifugation using the Bio-PRF system results in a larger PRF clot compared to fixed-angle centrifugation. One possible explanation is the variance in g-force distribution between fixed-angle and horizontal centrifugation. This variance led to marginally smaller quantities of PRF clots in the second tube batch, albeit not significantly so. Conclusions: In conclusion, the appearance and methodology of the two types of PRF we obtained are highly similar but not identical, with disparities in PRF clot quantity and consequently, in cellular properties. Notably, H-PRF exhibited a greater clot quantity compared to L-PRF.