Radish (<i>Raphanus sativus</i> L.) as an object of <i>Agrobacterium</i>-mediated transformation

Radish (Raphanus sativus L.) is an annual root crop closely related to Arabidopsis thaliana. It can be widely used as a perspective model plant to study the genetic control of storage root development. Thus, obtaining stable genetic transformants of radish to study the function of genes involved in storage root development becomes an important task. In this review, we present the currently available data on Agrobacterium-mediated transformation methods for radish, including in vitro and in planta methods using Agrobacterium tumefaciens, and a method for the production of composite plants with transformed roots or even whole transgenic plants with Agrobacterium rhizogenes. Improvement of existing methods and development of new approaches for radish transformation will increase the productivity of radish varieties, and the wider use of this promising model plant will provide new data on the genetic control of storage root development.

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Publication Details

Journal
Ecological genetics
Published
2024-11-28
DOI
https://doi.org/10.17816/ecogen640859
Primary Topic
Plant tissue culture and regeneration
Type
article
Field-Weighted Citation Impact
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article

Radish (Raphanus sativus L.) as an object of Agrobacterium-mediated transformation

И. Е. Додуева, Xenia A. Kuznetsova, Л. А. Лутова
Ecological genetics
Plant tissue culture and regeneration
article

Radish (Raphanus sativus L.) as an object of Agrobacterium-mediated transformation

И. Е. Додуева, Xenia A. Kuznetsova, Л. А. Лутова
article en

Abstract

Radish (Raphanus sativus L.) is an annual root crop closely related to Arabidopsis thaliana. It can be widely used as a perspective model plant to study the genetic control of storage root development. Thus, obtaining stable genetic transformants of radish to study the function of genes involved in storage root development becomes an important task. In this review, we present the currently available data on Agrobacterium-mediated transformation methods for radish, including in vitro and in planta methods using Agrobacterium tumefaciens, and a method for the production of composite plants with transformed roots or even whole transgenic plants with Agrobacterium rhizogenes. Improvement of existing methods and development of new approaches for radish transformation will increase the productivity of radish varieties, and the wider use of this promising model plant will provide new data on the genetic control of storage root development.

Ecological geneticsVol. 22(4)
St Petersburg University (RU)
Openalex Percentile: Top 81%
Plant tissue culture and regeneration
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