Amide conjugates of the jasmonate precursor <i>cis</i>-(+)-12-oxo-phytodienoic acid regulate its homeostasis during plant stress responses
Jasmonates are a family of oxylipin phytohormones regulating plant development and growth and mediating "defense versus growth" responses. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-OPDA) acts independently of CORONATIVE INSENSITIVE 1-mediated JA signaling in several stress-induced and developmental processes. However, its perception and metabolism are only partially understood. An isoleucine analog of the biologically active JA-Ile, OPDA-Ile, was detected years ago in wounded leaves of flowering plants, opening up the possibility that conjugation of cis-OPDA to amino acids might be a relevant mechanism for cis-OPDA regulation. Here, we extended the analysis of amino acid conjugates of cis-OPDA and identified naturally occurring OPDA-Val, OPDA-Phe, OPDA-Ala, OPDA-Glu, and OPDA-Asp accumulating in response to biotic and abiotic stress in Arabidopsis (Arabidopsis thaliana). The OPDA amino acid conjugates displayed cis-OPDA-related plant responses in a JA-Ile-dependent manner. We also showed that the synthesis and hydrolysis of cis-OPDA amino acid conjugates are mediated by members of the amidosynthetase GRETCHEN HAGEN 3 and the amidohydrolase INDOLE-3-ACETYL-LEUCINE RESISTANT 1/ILR1-like families. Thus, OPDA amino acid conjugates function in the catabolism or temporary storage of cis-OPDA in stress responses instead of acting as chemical signals per se.
Authors
- Takuya Kaji (ORCID: https://orcid.org/0000-0001-9633-5208)
- Jitka Široká (ORCID: https://orcid.org/0000-0002-4988-2919)
- Markéta Pernisová (ORCID: https://orcid.org/0000-0002-5803-2879)
- Ondřej Novák (ORCID: https://orcid.org/0000-0003-3452-0154)
- Vladimíra Nožková (ORCID: https://orcid.org/0000-0003-2862-8484)
- Michaela Králová (ORCID: https://orcid.org/0000-0002-5273-5936)
- Václav Mik (ORCID: https://orcid.org/0000-0002-8515-3035)
- Anita Ament (ORCID: https://orcid.org/0000-0001-5563-7330)
- Michal Karády (ORCID: https://orcid.org/0000-0002-5603-706X)
- Tomáš Pospíšil (ORCID: https://orcid.org/0000-0003-3634-828X)
- Federica Brunoni (ORCID: https://orcid.org/0000-0003-1497-9419)
- Chao Zhang (ORCID: https://orcid.org/0000-0002-4173-2107)
- Miroslav Strnad (ORCID: https://orcid.org/0000-0002-2806-794X)
- Claus Wasternack (ORCID: https://orcid.org/0000-0002-9872-6998)
- Rina Saito
- Kristýna Floková (ORCID: https://orcid.org/0000-0002-5558-0669)
- Minoru Ueda (ORCID: https://orcid.org/0000-0002-2305-3303)
- Mohamed Htitich (ORCID: https://orcid.org/0000-0002-8732-6286)
- Yuho Nishizato
Institutions
- Tohoku University (JP)
- Central European Institute of Technology – Masaryk University (CZ)
- Leibniz Institute of Plant Biochemistry (DE)
- Czech Academy of Sciences, Institute of Experimental Botany (CZ)
- Palacký University Olomouc (CZ)
Publication Details
- Journal
- PLANT PHYSIOLOGY
- Published
- 2024-11-28
- DOI
- https://doi.org/10.1093/plphys/kiae636
- Citations
- 12
- Primary Topic
- Insect-Plant Interactions and Control
- Type
- article
- Field-Weighted Citation Impact
- 6.48
Funders
- European Molecular Biology Organization
- Japan Society for the Promotion of Science London
- Ministry of Education, Culture, Sports, Science and Technology
- Grantová Agentura České Republiky
- Japan Society for the Promotion of Science