Genetically encoded bioluminescent glucose indicator for biological research
Glucose is an essential energy source in living cells and is involved in various phenomena. To understand the roles of glucose, measuring cellular glucose levels is important. Here, we developed a bioluminescent glucose indicator called LOTUS-Glc. Unlike fluorescence, bioluminescence doesn't require excitation light when imaging. Using LOTUS-Glc, we demonstrated drug effect evaluation, concurrent use with the optogenetic tool in HEK293T cells, and the measurement of light-dependent glucose fluctuations in plant-derived protoplasts. LOTUS-Glc would be a useful tool for understanding the roles of glucose in living organisms.
Authors
- Kazunori Sugiura
- Mitsuru Hattori (ORCID: https://orcid.org/0000-0003-1189-6554)
- Kenji Osabe (ORCID: https://orcid.org/0000-0002-5216-1055)
- Takeharu Nagai (ORCID: https://orcid.org/0000-0003-2650-9895)
- Ryo Tanaka
Institutions
- The University of Osaka (JP)
Publication Details
- Journal
- Biochemical and Biophysical Research Communications
- Published
- 2024-11-28
- DOI
- https://doi.org/10.1016/j.bbrc.2024.151092
- Citations
- 2
- Primary Topic
- bioluminescence and chemiluminescence research
- Type
- article
- Field-Weighted Citation Impact
- 0.47
Funders
- Takeda Science Foundation
- Uehara Memorial Foundation
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science
- Japan Science and Technology Agency
- Core Research for Evolutional Science and Technology