Visualizing Dynamic Weak Interaction Networks of Fluorine Atoms within Proteins via NMR
The utilization of fluorine probes in protein research has advanced our understanding of the nature of macromolecules. The diverse activities of proteins are governed by intricate weak interaction networks, yet the experimental detection presents a challenge. Herein, we have developed an NMR analytical method based on quantum coherent operations to characterize the weak chemical bonds of fluorine atoms within proteins that are bound to metal fluorides or labeled with fluorinated amino acids. Our approach offers a fast-screening method for identifying a weak interaction network without requiring crystallization.
Authors
- Maili Liu (ORCID: https://orcid.org/0000-0002-9359-915X)
- Zhaofei Chai
- Ling Jiang (ORCID: https://orcid.org/0000-0002-8505-8679)
- Zhou Gong (ORCID: https://orcid.org/0000-0001-6883-4026)
- Conggang Li (ORCID: https://orcid.org/0000-0002-5798-1722)
- Shixia Ji (ORCID: https://orcid.org/0009-0003-9444-3928)
- Yixiang Liu (ORCID: https://orcid.org/0000-0003-2977-1741)
- Wenqing Xia
Institutions
- Chinese Academy of Sciences (CN)
- Wuhan Institute of Physics and Mathematics (CN)
- Wuhan National Laboratory for Optoelectronics (CN)
- University of Chinese Academy of Sciences (CN)
- Huazhong University of Science and Technology (CN)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2024-11-27
- DOI
- https://doi.org/10.1021/acs.analchem.4c04773
- Citations
- 1
- Primary Topic
- Molecular spectroscopy and chirality
- Type
- article
- Field-Weighted Citation Impact
- 0.24
Funders
- National Natural Science Foundation of China
- Chinese Academy of Sciences