Study of toxicological parameters in laboratory animals under the influence of hypochloritic acid in a chronic experiment

The article presents materials on studying toxicological parameters in laboratory animals under hypochlorite acid obtained by electrolysis. It was established that the intra-gastric administration of a solution of hypochlorous acid in a concentration of 50 and 150 mg/l does not lead to systemic disturbances in the functional state of the most important organs and systems of the body and does not cause a decrease in the level of physical capabilities in experimental animals. At the same time, using a concentration of 300 mg/l caused a slight reduction in the physical capabilities of the experimental animals; namely, the swimming time decreased by 18.2 % compared to animals of the control group. With repeated administration of hypochlorous acid at a concentration of 300 mg/l, the absence of spontaneous “atypical” behavioral reactions was established, which could serve as important markers of stress or indicate severe behavioral and neurological disorders on the part of the central nervous system. Functional indicators of vertical and especially horizontal activity indicated regular physiological activity and emotional reaction, determined by the number of intersected squares in the center of the field and the time the animals were in the central squares. The experimental rats did not differ from the animals of the control group in terms of general psychophysiological parameters, the level of motor activity, tentative-exploratory reaction, the degree of their aggressive reaction to a change in the environment, and the absence of gait disturbances indicated the absence of changes in skeletal muscle tone. According to the “Straub Test”, the tone of experimental rats' tail muscles” stated a sufficient degree of excitation of the spinal motoneurons responsible for its regulation. The frequency of grooming and the number of fecal boluses in the experimental rats were within the physiological norm in frequency, quantity, and consistency and did not differ from the animals of the control group, indicating the absence of passive fear. The studied solution of hypochlorous acid at a concentration of 1000 mg/l did not cause signs of skin irritation, did not lead to the development of irritant reactions and non-allergic contact dermatitis in laboratory rats, and did not show an irritating, dermonecrotic, and resorptive effect on the skin of rabbits. Installation of a solution of hypochlorous acid at a concentration of 50 mg/l, in a volume of 0.05 cm3, into the conjunctival sac of a rabbit's eye did not cause an irritating effect on the mucous membrane.