Plasma DNA Methylation‐Based Biomarkers for MPNST Detection in Patients With Neurofibromatosis Type 1
Malignant peripheral nerve sheath tumor (MPNST) development is characterized by an altered DNA methylation landscape, which presents a promising area for developing MPNST-specific biomarkers for screening patients with NF1. Genome-wide DNA methylation profiling of a cohort of 13 patients with MPNST (29 samples of tumor and adjacent neurofibroma tissues) and of NF1-MPNST cell lines was performed to identify and validate candidate MPNST-specific CpG sites (CpGs). A logistic regression prediction model was constructed to select MPNST-specific CpGs distinct from adjacent neurofibromas and normal tissues. To test if hypermethylation at selected CpGs can also be detected in plasma from patients with MPNST, cfMBD-seq was applied to profile the cfDNA methylome of blood from patients with MPNST and NF1. Based on stringent feature-selection criteria and predictive modeling, we identified 73 candidate MPNST-specific CpGs (67 with unique CpG island locations) that reliably discriminated MPNSTs from neurofibromas. Validation of five candidate biomarkers confirmed successful MPNST detection (sensitivity: > 88%, specificity: > 91%) in tissues. In plasma samples, 63 of 67 selected genomic regions had greater than 1.2-fold higher methylation in patients with MPNST than those with NF1. Further, we identified 15 CpG islands that consistently separated plasma from patients with confirmed MPNST diagnosis from plasma of individuals with NF1 without a diagnosis of malignant transformation (FDR < 0.1). Our findings confirmed a unique hypermethylation pattern present during malignant transformation. This study highlights the potential to be investigated further as biomarkers in clinical settings for early MPNST detection in patients with NF1.
Authors
- Heather A. Lillemoe (ORCID: https://orcid.org/0000-0002-8732-7764)
- Ian E. McCutcheon (ORCID: https://orcid.org/0000-0002-1638-3400)
- Christopher P. Scally
- Kelly K. Hunt (ORCID: https://orcid.org/0000-0001-9156-8723)
- Justin Wong (ORCID: https://orcid.org/0000-0002-6803-1060)
- John M. Slopis (ORCID: https://orcid.org/0000-0002-1847-0583)
- Heather Lyu (ORCID: https://orcid.org/0000-0001-7759-0799)
- Jian Gu (ORCID: https://orcid.org/0000-0003-3499-0973)
- Alexander J. Lazar (ORCID: https://orcid.org/0000-0002-6395-4499)
- B. Ashleigh Guadagnolo (ORCID: https://orcid.org/0000-0002-4489-7070)
- Paul Scheet (ORCID: https://orcid.org/0000-0002-5173-7497)
- Sharon M. Landers (ORCID: https://orcid.org/0000-0001-9044-5510)
- Jace P. Landry (ORCID: https://orcid.org/0000-0001-7119-7005)
- S. Carson Callahan (ORCID: https://orcid.org/0000-0002-0152-2666)
- Keila E. Torres (ORCID: https://orcid.org/0000-0002-1855-0989)
- Kunal Rai (ORCID: https://orcid.org/0000-0003-2321-6894)
- Katarzyna Tomczak (ORCID: https://orcid.org/0000-0003-0553-8056)
- Emily Z. Keung (ORCID: https://orcid.org/0000-0002-8783-8484)
- Manishkumar S. Patel (ORCID: https://orcid.org/0000-0002-2450-0662)
- Christina Lynn Roland (ORCID: https://orcid.org/0000-0003-3400-0087)
- J. Andrew Livingston (ORCID: https://orcid.org/0000-0002-1337-3282)
- Angela D. Bhalla (ORCID: https://orcid.org/0000-0001-8863-1066)
- Christine B. Peterson (ORCID: https://orcid.org/0000-0003-3316-0468)
- Liang Wang (ORCID: https://orcid.org/0000-0002-9364-8572)
- Di Zhang (ORCID: https://orcid.org/0000-0001-5196-4633)
Institutions
- The University of Texas MD Anderson Cancer Center (US)
- Moffitt Cancer Center (US)
Publication Details
- Journal
- Molecular Carcinogenesis
- Published
- 2024-11-26
- DOI
- https://doi.org/10.1002/mc.23825
- Citations
- 5
- Primary Topic
- Neurofibromatosis and Schwannoma Cases
- Type
- article
- Field-Weighted Citation Impact
- 1.85
Funders
- Sally M. Kingsbury Sarcoma Research Foundation
- National Institutes of Health
- University of Texas MD Anderson Cancer Center
- National Cancer Institute