Immunostimulatory Effects of Red Beet Ferment Extract on Cyclophosphamide-Induced Immunosuppression in Wistar Rats

Red beet extract contains a variety of bioactive compounds, including antioxidant and immune-stimulating properties. Red beets are rich in nutrients and have been reported to help mitigate cardiovascular disease, while also possessing potent antioxidant capacity. In this study, we sought to determine the immune-enhancing effects of red beet fermented extract. The immunosuppressive drug cyclophosphamide (Cy) and red beet fermented extract were orally administered to Wistar rats once daily for four weeks. Blood analysis was performed to measure the expression of immune cytokines. We also examined the immunostimulatory effects of red beet fermented extract by measuring the activity and viability of spleen cells and the expression of immune cytokines. The expression of immune cytokines in the blood and spleen cells was increased in the red beet fermented extract treatment group compared to that in the negative control group, which induced immunosuppression of cytokines. It also increased the activity of NK cells and improved the viability of spleen cells. In conclusion, red beet fermented extract increased the expression of immune cytokines and increased the activity of adipocytes and NK cells, confirming its immunostimulatory effect. Therefore, red beet fermented extract has the potential to be utilized as a nutraceutical ingredient to enhance immunity.