Mechanisms of S. agalactiae promoting G. vaginalis biofilm formation leading to recurrence of BV

Previous research has established that the formation of Gardnerella vaginalis (GV) biofilm is one of the primary reasons for bacterial vaginosis (BV) recurrence. This study was the first to explore the impact of Streptococcus agalactiae (group B Streptococcus, GBS) on GV biofilm in a co-culture scenario. The results revealed that GBS could significantly increased the GV biomass in 48-hours dual-species biofilms. The luxS gene of GBS was significantly higher in dual-species biofilm, while knockdown of the luxS gene resulted in a significant decrease in mono- and dual-species biofilms. Meanwhile, in vitro addition of AI-2 (product of luxS gene) substantially increased biofilm biomass. Furthermore, we found that the expression of two genes related to biofilm formation was notably elevated in GV after receiving AI-2 signals. Collectively, these findings suggest that GBS enhances GV biofilm formation via luxS/AI-2 in an in vitro co-culture model, which in turn may promotes recurrence of BV.

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Publication Details

Journal
npj Biofilms and Microbiomes
Published
2024-11-27
DOI
https://doi.org/10.1038/s41522-024-00601-w
Citations
5
Primary Topic
Reproductive tract infections research
Type
article
Field-Weighted Citation Impact
1.72

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article

Mechanisms of S. agalactiae promoting G. vaginalis biofilm formation leading to recurrence of BV

Qingyun Jiang, Qinping Liao, Yifan Wu, Shuai Liu et al.
5 citations
npj Biofilms and Microbiomes
Reproductive tract infections research
1.72
article

Mechanisms of S. agalactiae promoting G. vaginalis biofilm formation leading to recurrence of BV

Qingyun Jiang, Qinping Liao, Yifan Wu, Shuai Liu, Jun Liu, Ying Liu, Lei Zhang, Hongbo Wei, Zhen Zeng, Xiaoqian Wang, Meng Li, Juhyun Kim, Simin Zhu, Ke Zhang
article en
5 citations

Abstract

Previous research has established that the formation of Gardnerella vaginalis (GV) biofilm is one of the primary reasons for bacterial vaginosis (BV) recurrence. This study was the first to explore the impact of Streptococcus agalactiae (group B Streptococcus, GBS) on GV biofilm in a co-culture scenario. The results revealed that GBS could significantly increased the GV biomass in 48-hours dual-species biofilms. The luxS gene of GBS was significantly higher in dual-species biofilm, while knockdown of the luxS gene resulted in a significant decrease in mono- and dual-species biofilms. Meanwhile, in vitro addition of AI-2 (product of luxS gene) substantially increased biofilm biomass. Furthermore, we found that the expression of two genes related to biofilm formation was notably elevated in GV after receiving AI-2 signals. Collectively, these findings suggest that GBS enhances GV biofilm formation via luxS/AI-2 in an in vitro co-culture model, which in turn may promotes recurrence of BV.

npj Biofilms and MicrobiomesVol. 10(1)
China Academy of Space Technology (CN), Beijing Tsinghua Chang Gung Hospital (CN), Tsinghua University (CN)
National Natural Science Foundation of China, Tsinghua University
Zero hunger
Openalex Percentile: Top 15%
Reproductive tract infections research
1.72
DOIOpen Access PDFOpenAlex
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