Imaging Human Pancreatic Endocrinogenesis During Early Prenatal Life
Data on human pancreas development are limited and derived from two-dimensional staining. We overcome this using in toto staining, tissue clearing, and light-sheet imaging. We sought to understand when and where endocrine cells first emerge and how they cluster. First, endocrine cell types appear simultaneously, and early pancreases contain bihormonal cells. There are morphometric differences between fetal and adult islets. We propose a mechanism of adult islet formation by fusion: a new base to reconstitute in vitro islet neogenesis.
Authors
- Raphaël Scharfmann (ORCID: https://orcid.org/0000-0001-7619-337X)
- Yorick Gitton (ORCID: https://orcid.org/0000-0002-6808-6010)
- Alain Chédotal (ORCID: https://orcid.org/0000-0001-7577-3794)
- Villalba Adrian
- Aiello Virginie
Institutions
- Université Claude Bernard Lyon 1 (FR)
- Centre National de la Recherche Scientifique (FR)
- Institut de la Vision (FR)
- Inserm (FR)
- École des Hautes Études en Santé Publique (FR)
- Université Paris Cité (FR)
- Sorbonne Université (FR)
- Hospices Civils de Lyon (FR)
- Institut de Recherche en Santé, Environnement et Travail (FR)
- Institut Cochin (FR)
Publication Details
- Journal
- Diabetes
- Published
- 2024-11-27
- DOI
- https://doi.org/10.2337/db24-0641
- Citations
- 6
- Primary Topic
- Pancreatic function and diabetes
- Type
- article
- Field-Weighted Citation Impact
- 3.44
Funders
- Canadian Institute for Advanced Research
- European Commission
- Agence Nationale de la Recherche
- Institut National de la Santé et de la Recherche Médicale