Development and application of a quadruplex TaqMan real-time fluorescence quantitative PCR assay for four porcine digestive pathogens
This quadruplex TaqMan qPCR assay offers a rapid, sensitive, and specific tool for the simultaneous detection of <i>Salmonella</i>, <i>Escherichia coli</i>, <i>Lawsonella intracellularis</i>, and <i>Brachyspira hyodysenteriae</i> in pigs.
Authors
- Zhi‐Min Jin (ORCID: https://orcid.org/0000-0001-5628-3917)
- Tongqing An
- Changyou Xia (ORCID: https://orcid.org/0000-0002-2701-1143)
- Caixia Gao (ORCID: https://orcid.org/0000-0003-3169-8248)
- Wei Wang (ORCID: https://orcid.org/0000-0001-6777-2441)
- Yue Sun (ORCID: https://orcid.org/0000-0003-0564-6380)
- He Zhang (ORCID: https://orcid.org/0000-0001-9198-6503)
- Liangquan Zhu
- Hongyan Chen (ORCID: https://orcid.org/0000-0001-9477-2260)
- Yue Wang (ORCID: https://orcid.org/0000-0003-1575-3569)
- Changqing Yu (ORCID: https://orcid.org/0000-0003-1311-3306)
- Jianxing Chen (ORCID: https://orcid.org/0000-0002-2363-9005)
- Haibo Yu (ORCID: https://orcid.org/0000-0002-7471-7923)
- Haojie Wang (ORCID: https://orcid.org/0009-0002-3260-811X)
Institutions
- Mudanjiang Normal University (CN)
- China Institute of Veterinary Drug Control (CN)
- Yibin University (CN)
- Chinese Academy of Agricultural Sciences (CN)
- Yibin Vocational and Technical College
- Harbin Veterinary Research Institute (CN)
Publication Details
- Journal
- Frontiers in Cellular and Infection Microbiology
- Published
- 2024-11-26
- DOI
- https://doi.org/10.3389/fcimb.2024.1468783
- Citations
- 4
- Primary Topic
- Viral gastroenteritis research and epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 1.56
Funders
- National Natural Science Foundation of China