1H MR spectroscopy and IVIM-DWI to evaluate the effect of a choline kinase inhibitor and temozolomide therapy in a mouse model of glioblastoma

Motivation: There is a lack of comprehensive understanding regarding the impact of choline kinase inhibition and standard chemotherapy on preclinical GBM models. Goal(s): The primary goal of this study was to elucidate the treatment response mechanism in a preclinical GBM mouse model. Approach: MRS and IVIM-DWI were used for monitoring metabolic and microstructural changes in a preclinical GBM model by single or combination therapy with a choline kinase inhibitor and TMZ. Results: Our findings indicate that the combination therapy is the most effective treatment regimen. This study contributes to a better understanding of treatment response mechanisms and underscores the potential of non-invasive MRI methods. Impact: Targeting ChoKα inhibition and the damage of DNA replication (TMZ) promises to be an alternative in the treatment of GBM. This research also highlights the importance of MRS and IVIM-DWI as promising non-invasive methods to assess therapeutic effects on GBM.