Impact of jugular vein ligation on cerebrospinal fluid clearance from G-lymphatic system in mice

Motivation: Cerebral venous outflow abnormalities have been linked to various neurological disorders, necessitating a detailed understanding of their impact on brain and lymphatic perfusion. This study aimed to investigate the G-lymphatic system change following bilateral jugular vein ligation (JVL) in mice. Goal(s): Deeper understanding of the venous system's role in CNS fluid homeostasis. Approach: JVL was performed in mice. 2D-TOF, DCE-FLASH and 3D-MGE imaging were acquired at baseline, 2, 7 and 14-days post-surgery. Quantitative analysis was used to assess changes in lymphatic flow, brain volumetry. Results: JVL induced hypertension, bigger brain and veinous system. The permeability in the brain reduced before returning to baseline. Impact: Our study demonstrated progressive alterations in cerebral blood flow in mice following jugular vein ligation, highlighting the utility of MRI for studying the G-lymphatic system in brain. These findings contribute to a better understanding of cerebrovascular changes in living conditions.