Investigation of Metabolic Heterogeneity in von Hippel Lindau (VHL) Patient Derived Renal Tumor cells using NMR Stable Isotope Tracing

Motivation: To develop targeted therapies for Clear Cell Renal Cell Carcinoma (ccRCC). Goal(s): Investigation of metabolic activity in von Hippel-Lindau (VHL) patient derived renal tumor cells using NMR based stable isotope tracing to develop targeted tumor therapies. Approach: Cultured VHL patient derived renal tumor cells (UOK270 and UOK 366) were harvested and extracted upon 24 h of [U-13C] Glucose tracing and analyzed using NMR based stable isotope Resolved Metabolomics (SIRM). Results: After 24 h of [U-13C] Glucose tracing, we determined the relative levels of 13C incorporation in Glycolysis and (Tri Carboxylic Acid) TCA cycle intermediates in UOK 270 and UOK366 using NMR based SIRM. Impact: This is the first-time investigation of metabolic activity in VHL patient derived renal tumor cells generated in house (UOK270 and UOK366) impacting on ccRCC treatment development.