Development of a dynamic contrast-enhanced MRI based acquisition and analysis method for investigating the glymphatic system in rats

Motivation: The glymphatic system’s role in metabolic waste removal in the brain is crucial. Impairment of the flow of cerebrospinal fluid (CSF) and interstitial fluid (ISF) has been linked to several neurological conditions. Goal(s): Our aim is to develop a method combining contrast media administration, MRI, and postprocessing for detailed study of the glymphatic system in rats. Approach: We investigated how administration routes with various invasiveness effect the distribution of contrast agent (CA) in the healthy rat brain and compared different data analysis methods. Results: Only cisterna magna application showed satisfying results. Time series cluster analysis shows patterns in the contrast agent distribution dynamic. Impact: A reliable MRI-based method for investigating the glymphatic system in rats will allow for the study of its role in several neurodegenerative and other diseases.