A simple, economical, and high-yield method for polyethylene glycol-based extraction of follicular and serum-derived extracellular vesicles
This study overcomes the time-consuming, expensive, laborious, and complicated machine-dependent EV extraction methods. The study highlights that longer incubation time is needed for EV extraction from FF. PEG 8000-based EV extraction provided a higher yield and less carry-over protein than ExoQ-based EV extraction.
Authors
- Pao‐Chu Chen (ORCID: https://orcid.org/0000-0003-2350-9873)
- Sung‐Chao Chu (ORCID: https://orcid.org/0000-0002-2737-0787)
- Tang‐Yuan Chu (ORCID: https://orcid.org/0000-0003-2717-2681)
- Aye Aye Khine (ORCID: https://orcid.org/0009-0005-9472-0950)
- Hsuan-Shun Huang
- Yu-Shuan Chen
- Ying-Hsi Chen
Institutions
- Tzu Chi University (TW)
- Tzu Chi Foundation (TW)
- Hualien Tzu Chi Medical Center (TW)
Publication Details
- Journal
- Tzu Chi Medical Journal
- Published
- 2024-10-25
- DOI
- https://doi.org/10.4103/tcmj.tcmj_160_24
- Citations
- 2
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.47
Funders
- Buddhist Tzu Chi Medical Foundation
- Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation