In vivo 1H MRS identifies intramyocellular lipids as early prediabetic marker in diet-induced obesity

Motivation: Obesity is one of the main risk factors for type 2 diabetes and is also associated with an increased cardiovascular risk. However, the transition from the early states of impaired glucose intolerance to a more progressive disease stage still remains elusive. Goal(s): To identify biomarkers and time points for early therapeutic interventions to stop further transition. Approach: We used an established mouse model exposed for 9 weeks to a diabetogenic diet and longitudinally monitored important organs/tissues by MRI+MRS. Results: Using MRS we could detect massive accumulation of lipids in all organs which preceded even a sigificant weight gain in DD-fed mice. Impact: MRS of intramyocellular lipids was most sensitive to reveal in vivo very early alterations in the prediabetic state and, thus, may also be used in humans to identify patients at the transition point from prediabetes to diabetes.