PET/MRI dual-model Granzyme B targeted probe for non-invasive early diagnosis of acute heart allograft rejection

Motivation: Early diagnosis of transplant rejection can help to improve the immune-related management of transplant recipients. Goal(s): Developing the new methods for early and non-invasive diagnosis of acute cellular rejection to unmet clinical needs. Approach: Develop a PET/MRI dual-model granzyme B (GzmB) targeted probe for non-invasively detection of transplant acute cellular rejection. Results: In preclinical heart graft models of rejection, our PET/MRI dual-model GzmB targeted probe allow noninvasive discrimination of early acute cellular rejection mediated by recipient cytotoxic CD8+ T cells. Impact: In the future, this technology developed a PET/MRI dual-model probe for imaging GzmB produced by cytotoxic CD8+ T cells, enabling early non-invasive diagnosis of allograft rejection with high sensitivity and high spatial resolution.