Energetic Metabolism during Preclinical Migraine Measured by 31P Spectroscopy at 21.1 T

Motivation: Migraine is a disorder of neuronal hyperexcitability, but previous work has not been able to capture energetic processes non-invasively during active central sensitization, only after or between migraine attacks. Goal(s): This preclinical study evaluates whole brain energetic metabolism and remodeling during a nitroglycerin-induced migraine attack. Approach: 31P spectroscopy using ISIS was used to measure phosphocreatine and ATP levels at baseline and then over a 3-hour period post-NTG administration. Results: Most notable is the increase in phosphocreatine compared to baseline and controls, as well as an increase in ATP compared to baseline. Both indicate energetic remodeling during central sensitization prior to migraine pain onset. Impact: Increased phosphocreatine and ATP over the entire brain demonstate significant energetic fluxes during cerebral central sensitization and prior to nociception. This energetic remodeling informs understanding of fundamental migraine pathophysiology and its timing, potentially impacting the administration of potential clinical interventions.