Refinement of the model and types of data for quantitative Magnetization Transfer (qMT) in brain tissues

Motivation: To improve the accuracy of quantitative Magnetization Transfer (qMT) for interrogation of brain microstructure. Goal(s): To determine: i) the model for qMT that best describes the signal from MT and inhomogeneous MT (ihMT) experiments, and ii) the MT preparations that contribute useful data. Approach: We tested accuracies of the models based on fit quality in-vivo and ex-vivo, comparing ex-vivo qMT at physiological and room temperatures. Data were retrospectively reduced to test the importance of types of MT preparations. Results: We recommend a model with two bound-pool T2 values and use of different MT pulse widths and duty cycles as a result. Impact: Our results will impact the model and data acquired for quantitative Magnetization Transfer (qMT) of brain tissues. Use of a two bound-pool model with distinct T2 values and data inputs with variations in MT pulse width and DC is recommended.