ECS-induced seizure enhances neurometabolic activity but doesn’t affect astrocytic activity in mouse brain

Motivation: Electroconvulsive shock (ECS) therapy is an established treatment for drug-resistant depression. However, the status of neurometabolic activity during ECS-induced seizure is not clear. Goal(s): This study was carried out to measure neurometabolic activity during ECS-induced Seizure. Approach: 1H-[13C]-NMR spectroscopy was used in conjunction with intravenous [1,6-13C2]glucose or [2-13C]acetate infusion to measure neuronal and astroglial metabolic activity, respectively. Results: The neurometabolic activity was increased while astroglial activity remained unchanged during ECS-induced seizure. Moreover, the anaerobic glucose consumption was highly increased, suggesting the dominance of glycolysis during the seizure state. Impact: The assessment of metabolic activity of Glutamatergic, GABA’ergic neurons, and astrocytes during ECS-induced seizures, provides valuable information about glucose consumption during enhanced energy demand, and the interplay of different cell types in the brain.