Unveiling Metabolite Alterations in Photon-Irradiated Tramp C1 Cells via Hyperpolarized 13C NMR

Motivation: Investigating metabolic changes in glycolysis within Tramp C1 cells due to photon irradiation, focusing on the role of HP 13C pyruvate. Goal(s): To determine how photon irradiation affects pyruvate-to-lactate flux in vitro, indicating metabolic reprogramming. Approach: Utilizing benchtop NMR to monitor HP 13C dynamics in irradiated versus control cells, targeting lactate production. Results: Photon irradiation elevated 13C lactate production by two-fold on Day 0, with persistently higher levels observed on Day 3, signifying altered metabolic flux post-irradiation. Impact: This study's findings reveal photon irradiation's potent effect on cellular metabolism, potentially influencing radiation therapy strategies. It opens avenues for targeted metabolic interventions and underscores the metabolic resilience in Tramp C1 cells, crucial for cancer treatment optimization.