Shear-induced Sodium Dynamics within Red Blood Cells membrane using advanced 23Na Single and Triple Quantum Filtered Rheo-NMR methods.

Motivation: Increased blood viscosity has been reported in red blood cell senescence and pathologies of decreased perfusion such as chronic kidney disease. Goal(s): Rheological NMR is a powerful, although still underutilized, tool to assess the molecular-mechanical links in flowing media. This study's primary objective was to explore the dynamic alterations in Na+ interaction with glycocalyx of red blood cells exposed to the physiological shear forces experienced within the microvasculature in health and disease.Approach: 23Na multiple quantum filtered (MQF) rheo-NMR in RBCs suspensions at varied haematocrit. Results: 23Na MQF rheo-NMR are efficient to detect Na+ interaction with glycocalyx of red blood cells under deformationImpact: Potential sodium “rheo-markers” reflective of blood viscosity in health and disease are proposed.