Time-dependent response of Silver nanoparticles reinforced biocomposite in Murine fibroblast cells by NMR-based Pharmacometabolomics

Motivation: Conventional toxicity assays generally convey the efficiency of nanomaterial, but they lack a mechanistic molecular approach to toxicity. Goal(s): We aimed to employ NMR-based Pharmacometabolomics to detect early metabolic alterations associated with nanomaterial developed for healthcare. Approach: The NMR spectroscopy technique was used on Silver nanoparticles reinforced biocomposite exposed murine fibroblast (L929) cells. Results: Results demonstrated that cellular metabolic profiles reset towards control upon more exposure. Impact: Our nanomaterial-based study accentuates the role of pharmacometabolomics, which will complement the findings of conventional assays, eventually leading to efficacious clinical translation.