Observation of morphochemical changes and water molecule dynamics of Trypoxylus dichotomus in the pupal stage using 9.4-T MRI

Motivation: Clarification of tissue destruction and reconstruction process of holometabolous insects might lead to innovative technology for regenerative medicine.Goal(s): Observation of Morpholochemical changes and water molecule dynamics in the pupal body. Approach: We applied T2W, DTI and PRESS to the pupal body of Trypoxylus dichotomus.Results: From the pupation, the digestive tract swelled and became a liquid reservoir. It has high ADC and FA in the Head-Foot direction. After the pupation, the structure was narrower and elongated. The results suggest that destructed tissues were stored temporarily in the form of reservoir, and through the liquid channel around the reservoir to reconstruction tissues.Impact: In the pupal body, the tissue absorbed into the digestive tract while some of the nervous system and muscles are retained formed a liquid reservoir, used to form adult tissues such as flight muscles through certain liquid channel.