Characterizing Tissue Relaxation and Magnetization Transfer in Fresh, Thawed, and Fixed White Matter Tissue Samples
Motivation: Studying ex vivo tissue requires preservation by formalin-fixation or freezing. Effects of these methods on tissue parameters compared to fresh tissue is unknown. Goal(s): We investigated how freezing/thawing and fixation affect T1, T2, and MT properties in brain tissue. We created a protocol to apply MR methods (T2-MESE, biexponential T1, qMT, ihMT, NODDI) to pathology specimens in the Michigan Alzheimer’s Disease Research Center (MADRC) repository. Approach: We scanned the same ex vivo sheep brain samples fresh, frozen/thawed, and fixed, and compared their relaxation and MT properties. Results: Effects of fixation are most prominent in white matter and especially influence T1 and T2 relaxation. Impact: Thawed tissue exhibits more similar relaxation and MT properties to fresh tissue than fixed tissue does. In MR studies that use ex vivo tissue samples, such as those correlating MR to histology, thawed tissue may be preferable to formalin-fixed.
Authors
- Steven Swanson (ORCID: https://orcid.org/0000-0002-5896-1037)
- Jon‐Fredrik Nielsen (ORCID: https://orcid.org/0000-0002-2058-3579)
- Jeffrey A. Fessler (ORCID: https://orcid.org/0000-0001-9998-3315)
- Navid Seraji‐Bozorgzad (ORCID: https://orcid.org/0000-0002-8215-6513)
- Ulrich M. Scheven (ORCID: https://orcid.org/0000-0001-8111-0081)
- Amaya Murguia (ORCID: https://orcid.org/0009-0004-5546-1274)
Publication Details
- Journal
- Proceedings on CD-ROM - International Society for Magnetic Resonance in Medicine. Scientific Meeting and Exhibition/Proceedings of the International Society for Magnetic Resonance in Medicine, Scientific Meeting and Exhibition
- Published
- 2024-11-26
- DOI
- https://doi.org/10.58530/2024/2036
- Citations
- 1
- Primary Topic
- Advanced MRI Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.40