Blood brain barrier permeability characterization in the NOD-EAE mouse model of secondary progressive multiple sclerosis using MRI

Motivation: Blood brain barrier (BBB) dysregulation is one of the earliest signs of multiple sclerosis (MS) and the mechanism underlying BBB breakdown is not completely understood. Goal(s): We sought to use the non-obese diabetic experimental allergic encephalomyelitis (NOD-EAE) mouse model of secondary progressive MS to understand BBB breakdown in efforts to explore potential MS therapeutics. Approach: MRI was used to quantify BBB permeability metrics using gadolinium contrast agent. Results: We quantified the spatial and temporal characterization of BBB permeability in NOD-EAE mice with progressing disease using MRI. These quantifying parameters can potentially be used to test the effect of therapeutic agents on BBB breakdown. Impact: The NOD-EAE mouse model of secondary progressive multiple sclerosis (SPMS) can potentially be used to assess blood brain barrier characteristics using contrast-enhanced MRI in efforts to test therapeutic agents that can be used in the treatment of SPMS.