MICROCLONAL REPRODUCTION OF EDIBLE HONEYSUCKLE AND ITS VEGETATIVE DEVELOPMENT IN VITRO CONDITIONS

Honeysuckle (Lonicera edulis) is a valuable plant with extreme early maturity - its fruits ripen 7-10 days earlier than strawberries in the conditions of the Republic of Tatarstan. Currently, there is a great demand for seedlings of fruit and berry crops in Russia. This is due to the increase in areas in industrial, homestead and collective gardening. Having conducted statistics on agricultural mortgages, we identified a huge trend for families to return to private homesteads. Therefore, clonal micropropagation is the most effective method for obtaining a large amount of high-quality planting material to create not only industrial plantings, but also to provide the population with standard seedlings. At the stage of introduction into the culture in vitro, effective sterilization modes were selected, and at the stage of reproduction, the further development of micro plants in the culture was studied. During the rooting process, two varieties of edible honeysuckle were observed and the effect of auxin on root formation and growth of the above-ground part of the plant was studied. The most viable sterile explants were observed with successive sterilization in solutions of 0.1% KMnO4 (3 min), 7.5% "Belizna" (7 min) and 0.01% Miramistin (1 min). The yield of sterile explants in this case averaged 69.8%. The best results were obtained with the Assol variety with an above-ground part growth of 6.3 cm and root system formation of 19.6 cm. At the fourth stage of "Adaptation", no varietal differences in survival were found. Both varieties of edible honeysuckle (Peter 1 and Assol) showed high survival rates.

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Publication Details

Journal
Agrobiotechnologies and digital farming
Published
2024-11-26
DOI
https://doi.org/10.12737/2782-490x-2024-14-19
Primary Topic
Plant tissue culture and regeneration
Type
article
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article

MICROCLONAL REPRODUCTION OF EDIBLE HONEYSUCKLE AND ITS VEGETATIVE DEVELOPMENT IN VITRO CONDITIONS

Галина Абрамова, А. Г. Абрамов, Zifa Salihova
Agrobiotechnologies and digital farming
Plant tissue culture and regeneration
article

MICROCLONAL REPRODUCTION OF EDIBLE HONEYSUCKLE AND ITS VEGETATIVE DEVELOPMENT IN VITRO CONDITIONS

Галина Абрамова, А. Г. Абрамов, Zifa Salihova
article en

Abstract

Honeysuckle (Lonicera edulis) is a valuable plant with extreme early maturity - its fruits ripen 7-10 days earlier than strawberries in the conditions of the Republic of Tatarstan. Currently, there is a great demand for seedlings of fruit and berry crops in Russia. This is due to the increase in areas in industrial, homestead and collective gardening. Having conducted statistics on agricultural mortgages, we identified a huge trend for families to return to private homesteads. Therefore, clonal micropropagation is the most effective method for obtaining a large amount of high-quality planting material to create not only industrial plantings, but also to provide the population with standard seedlings. At the stage of introduction into the culture in vitro, effective sterilization modes were selected, and at the stage of reproduction, the further development of micro plants in the culture was studied. During the rooting process, two varieties of edible honeysuckle were observed and the effect of auxin on root formation and growth of the above-ground part of the plant was studied. The most viable sterile explants were observed with successive sterilization in solutions of 0.1% KMnO4 (3 min), 7.5% "Belizna" (7 min) and 0.01% Miramistin (1 min). The yield of sterile explants in this case averaged 69.8%. The best results were obtained with the Assol variety with an above-ground part growth of 6.3 cm and root system formation of 19.6 cm. At the fourth stage of "Adaptation", no varietal differences in survival were found. Both varieties of edible honeysuckle (Peter 1 and Assol) showed high survival rates.

Agrobiotechnologies and digital farmingVol. 3(3)
Kazan State Agrarian University (RU)
Openalex Percentile: Top 81%
Plant tissue culture and regeneration
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