The therapeutic effect of antioxidant postbiotic cocktail in colitis mice: A promising approach to alleviate oxidative stress in two high-fat and normal-diet feeding mice

It seems that postbiotics as a byproduct of fermentation of probiotic bacteria could be more effective in health benefits context. Therefore, in this study we aimed to assess the effectiveness of a novel postbiotic resulted from native probiotic Lactobacillus strains with robust antioxidant properties in ameliorating colitis in murine models fed with Normal-Diet or High Fat-Diet. Eighty-eight strains of Lactobacillus and Bifidobacterium sourced from human feces and milk were screened for antioxidant activity. Six potent strains were selected to formulate a postbiotic cocktail, with its major compounds identified through gas chromatography. In an animal study, mice with dextran sulphate sodium (DSS)-induced colitis that received the postbiotic cocktail showed significant improvements in phenotypical indices compared to the DSS group. Mice on both dietary regimens exhibited reduced adverse effects associated with DSS consumption, as indicated by lower Disease Activity Index (DAI) and Pathological Score metrics, along with increased intestinal mass and length (p<0.05) . Additionally, mice receiving the postbiotic cocktail demonstrated elevated levels of antioxidant enzymes and anti-inflammatory cytokines (p<0.05) . This suggests that the postbiotic not only mitigates inflammation but also enhances antioxidant defenses, particularly in those on a normal diet. Overall, these findings indicate that this novel postbiotic mixture has potential for effectively managing colitis, especially when combined with a healthy dietary regimen, while also highlighting opportunities to optimize environmental factors to enhance its antioxidant capabilities. • In colitis, oxidative stress triggers inflammation and depletes antioxidants. • Our novel postbiotic alleviated colitis induced by dextran sulphate sodium. • Novel postbiotic was more effective in Normal diet-fed mice. • Novel postbiotic alleviated colitis by modulating Nrf2 as an antioxidant pathway. • Novel postbiotic alleviated colitis by modulating NF-kB as an Inflammatory pathway.