Rapid test for hepatitis B core-related antigen to identify people living with hepatitis B having high viral load in Cameroon
This study presents a retrospective assessment of the diagnostic performance of the newly developed hepatitis B core-related antigen rapid diagnostic test (HBcrAg-RDT) in detecting plasma samples with elevated hepatitis B virus (HBV) DNA levels (≥200,000 IU/ml) in Yaoundé, Cameroon. Samples were collected consecutively from treatment-naïve adults living with HBV between January 1, 2021, and June 30, 2023. Analyzing 146 samples from participants with a median age of 36 years, the HBcrAg-RDT exhibited a sensitivity of 97.5% (95% CI: 86.8-99.9) and a specificity of 77.4% (68.2-84.9) when compared to real-time PCR as the reference standard. These findings suggest that HBcrAg-RDT holds promise as a valuable point-of-care tool for diagnosing high HBV DNA levels, particularly in resource-limited settings. Further research will refine its practicality and effectiveness.
Authors
- Naoki Yamamoto (ORCID: https://orcid.org/0009-0001-7364-9543)
- Jeanne Perpétue Vincent (ORCID: https://orcid.org/0000-0003-2448-2908)
- Tomoyuki Miura (ORCID: https://orcid.org/0000-0002-1956-2180)
- Yasuhito Tanaka (ORCID: https://orcid.org/0000-0002-2473-6966)
- Masato Ichikawa
- Yusuke Shimakawa (ORCID: https://orcid.org/0000-0002-4198-4785)
- Mari Nagai (ORCID: https://orcid.org/0000-0001-6731-5064)
- Katsumi Aoyagi (ORCID: https://orcid.org/0000-0003-3818-7985)
- Atsushi Kaneko (ORCID: https://orcid.org/0000-0003-4392-912X)
- Richard Njouom (ORCID: https://orcid.org/0000-0003-3112-6370)
- Wataru Sugiura (ORCID: https://orcid.org/0000-0002-3132-0918)
- Naofumi Hashimoto
- Frédéric Lissock
- Masaya Baba (ORCID: https://orcid.org/0000-0002-5308-6683)
- Abdou Fatawou Modiyinji (ORCID: https://orcid.org/0000-0001-6706-3012)
- Alassane Ndiaye
Institutions
- Institut Pasteur (FR)
- Kumamoto Health Science University (JP)
- Université Paris Cité (FR)
- National Center for Global Health and Medicine (JP)
- Centre Pasteur du Cameroun (CM)
- Fujirebio (Japan) (JP)
- Kumamoto University (JP)
Publication Details
- Journal
- Virology
- Published
- 2024-11-26
- DOI
- https://doi.org/10.1016/j.virol.2024.110316
- Citations
- 1
- Primary Topic
- Hepatitis B Virus Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.39
Funders
- Japan Agency for Medical Research and Development
- National Center for Global Health and Medicine
- Japan Society for the Promotion of Science