Antimicrobial Activity of Glycyrrhizinic Acid Is pH-Dependent

In recent years, antimicrobial hydrogels have attracted much attention in biomedical applications due to their biocompatibility and high water content. Glycyrrhizin (GA) is an antimicrobial that can form pH-dependent hydrogels due to the three carboxyl groups of GA that differ in p<i>K</i>_a value. The influence of GA protonation on the antimicrobial activity, however, has never been studied before. Therefore, we investigated the effect of the pH on the antimicrobial activity of GA against <i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i>, MRSA, <i>Staphylococcus epidermidis</i>, <i>Acinetobacter baumannii</i>, <i>Klebsiella pneumoniae</i>, <i>Klebsiella aerogenes</i>, and two strains of <i>Escherichia coli</i>. In general, the antimicrobial activity of GA increases as a function of decreasing pH (and thus increasing protonation of GA). More specifically, fully protonated GA hydrogels (pH = 3) are required for growth inhibition and killing of <i>E. coli</i> UTI89 and <i>Klebsiella</i> in the suspension above the hydrogel, while the staphylococci strains and <i>A. baumannii</i> are already inhibited by fully deprotonated GA (pH = 6.8). <i>P. aeruginosa</i> and <i>E. coli</i> DH5α showed moderate susceptibility, as they are completely inhibited by a hydrogel at pH 3.8, containing partly protonated GA, but not by fully deprotonated GA (pH = 6.8). The antimicrobial activity of the hydrogel cannot solely be attributed to the resulting pH decrease of the suspension, as the presence of GA significantly increases the activity. Instead, this increased activity is due to the release of GA from the hydrogel into the suspension, where it directly interacts with the bacteria. Moreover, we provide evidence indicating that the pH dependency of the antimicrobial activity is due to differences in GA protonation state by treating the pathogens with GA solutions differing in their GA protonation distribution. Finally, we show by LC-MS that there is no chemical or enzymatic breakdown of GA. Overall, our results demonstrate that the pH influences not only the physical but also the antimicrobial properties of the GA hydrogels.