Selection and validation of reference genes for qRT-PCR normalization in dayflower (Commelina communis) based on the transcriptome profiling

This study identified the most stably expressed reference genes under three abiotic stresses and in five organs of dayflower, and SAND showed high expression stability under various experimental conditions, making it a reliable reference gene for gene expression analysis experiments under different conditions in dayflower. This study will enhance the precision of the qRT-PCR quantification of candidate genes related to the adaptation significance of dayflower.