Multi-omics uncovers the potential functions of transcription factor Dp-1 in human digestive cancers

• A multi-omics analysis of the potential roles of TFDP1 in human digestive cancers was performed. • These findings can facilitate the development of TFDP1-related diagnostic and therapeutic strategies for digestive cancers. • The functions of another gene of interest in different cancers can be easily studied using this research flow. Previous studies have reported the critical effects of TFDP1 on the occurrence and progression of multiple cancers. Nevertheless, these studies were confined to a single cancer type rather than human digestive cancers. Therefore, using various integrated databases available, we conducted a multi-omics analysis of the potential roles of TFDP1 in digestive cancers. Firstly, high TFDP1 mRNA expression levels were observed in all seven digestive cancers, and were closely related to survival differences, pathological features, as well as immune and molecular subtypes. Epigenetic alterations of TFDP1 were evident in the four digestive cancers, and TFDP1 promoter methylation was negatively correlated with TFDP1 mRNA expression. Subsequently, using MuTarget, we identified several mutant genes accountable for TFDP1 overexpression. In addition, function enrichment analysis unveiled that TFDP1 could influence the cell cycle process and the pathways of the cell cycle and FoxO signaling. Intriguingly, our single-cell analysis demonstrated high expression of TFDP1 in blood and immune cells. We also found the statistically significant correlation of TFDP1 expression with six infiltration cell types, four immunosuppressive cell types, and various drugs. Finally, the TIDE results revealed that TFDP1 had a higher predictive ability of TFDP1 than four classical biomarkers and TFDP1 expression was associated with various types of cohorts. Taking the CRISPR screen cohorts, we observed the powerful effect of TFDP1-knockout on MC38 colon cancer cells. These findings could help elucidate the potential functions of TFDP1 and facilitate the development of TFDP1-related diagnostic and therapeutic strategies for different digestive cancers.