Chemo Metric Assisted Method Development for the Estimation of Paracetamol, Etoricoxib and Thiocolchicoside in Combined Formulation

Chemometric based analytical method was developed for the estimation of Paracetamol (PCM), Etoricoxib (ECB) and Thiocolchicoside (TCS) by UV-VIS spectrophotometry. Spectroscopic technique, a simple, rapid, precise and accurate process for amount estimation is still in great use for analyst. For estimation of these drugs, solvent alcohol 90% was utilised and 248.5nm, 287nm and 380.5nm were the wavelength of absorbance measurement for PCM, ECB and TCS respectively. Effect of input variables on spectrum characteristics were studied for selection of critical parameters and developed method was validated as per ICH Q 2 R1 regulatory guidelines. Linearity of all these three drugs was ascertained over the conc range 1-14μg/ml for PCM and 1-24 μg/ml for both ECB and TCS. The accuracy of assay was found 102.66% for PCM, 98.56% for ECB and 98.02 % for TCS; and the precision study was shown acceptable data as %RSD data varied from 0.5382 to 1.6227 for PCM, from 0.4280 to 1.0521 for ECB and from 0.1438 to 0.8553 for TCS. The developed method is rigid, robust and efficient for the estimation of PCM, ECB and TCS from the combined composition of dosage form.