Development of an HPLC Method for Simultaneous Quantitation of Hyperin, Quercetin and Kaempferol in the Semen cuscutae

In this study, a method for the simultaneous quantification of hyperin, quercetin, and kaempferol in samples of Semen cuscutae was developed using high-performance liquid chromatography (HPLC). The chosen chromatographic conditions were a mobile phase of methanol – 0.1% formic acid in water, running in gradient mode, with a flow rate of 0.9 ml/min, and detection wavelengths of 354, 365, and 369 nm for hyperin, kaempferol, and quercetin, respectively. The results of the quantitative analysis of 5 samples of Semen cuscutae purchased from Phu Tho province showed hyperin content ranging from 0.950 to 1.218 mg/g; quercetin from 0.077 to 0.118 mg/g; and kaempferol from 1.051 to 1.190 mg/g based on the dry weight of the medicinal material. This research may be suitable for the quality control of Semen cuscutae in the market.