Growth and Biochemical Characteristics of Microalgae Chlorella vulgaris Grown on Various Combinations of Fish Waste Hydrolysate and Seaweed Hydrolysate

Background: to utilize the fish waste and seaweed as a media for the growth of microalgae Chlorella vulgaris and carbon sequestration. Methods: Culture of Chlorella vulgaris in the fish waste and seaweed hydrolysate, analysed the cell density, biomass, chlorophyll, carotenoid content, carbon-di-oxide sequestration and proximate of microalgae (Protein, Carbohydrate, Lipid and Ash). Result: In the combination of fish acid hydrolysate and sargassum sp., acidhydrolysate, the respective peak values of cell density, average specific growth rate, biomass, chlorophyll a and b, carotenoid contentand carbon-di-oxide sequestrated recorded are 4.26±0.06×106 cells/ml, 0.58 µ/day, 1.98±0.04 g/l, 5.59±0.09 µg/ml and 3.69±0.04 µg/ml, 3.64±0.05 µg/ml, 3.73±0.07 g/l/d in 3:6 combination. Corresponding protein, carbohydrate, lipid and ash content observed in the resultant algae are 51.77±0.6%, 15.4±0.2%, 10±0.53 and 7.08±0.4% respectively.