Study of Chiral Center Effect on CaLB-Catalyzed Hydrolysis of (±)-1-(Acetoxymethyl)-3, 4, 5-methylpyrrolidin-2-ones

Several chemical and biocatalytic methods have been described for chiral γ-lactams syntheses. However, only one biocatalytic method has been reported for γ4-lactam resolution, while γ2- and γ3-lactams have not been reported. On the other hand, its resolution through biocatalysts is complicated since enzymes such as ENZA-1 (Rhodococcus equi NCIB 40213) and ENZA-20 (Pseudomonas solanacearum NCIB 40249) are difficult to obtain. Therefore, in this paper, the resolution of γ-lactams 7-9 was carried out through a hydrolysis reaction using the commercially available enzyme CaLB.