Memory Enhancing Activity of Combination Extracts of <i>Bauhinia variegata</i> Leaf and <i>Madhuca longifolia</i> Leaf by Elevated Plus Maze and Estimation of MDA, SOD and Catalase

The aim is to investigate the role of a combination of Madhuca longifolia (ML) and Bauhinia variegata (BV) ethanolic leaf extracts against oxidative damage in Swiss albino mice and colchicine-induced cognitive dysfunction; to estimate the neuroprotective impact using M. longifolia and B. variegata combination by behavioral testing and to analyze the biochemical parameters. The experimental analysis was done for 28 days on a colchicine-induced model. Elevated plus maze and actophotometer were used for behavioral experiments and biochemical parameters like SOD, catalase and MDA were analyzed. 36 Swiss albino mice were grouped into six groups, each consisting of six mice. 1 percent w/v Carboxy Methyl Cellulose (CMC) was administered to Group I. Group II received effective dose ED (BV) +ED (ML) (400mg/ kg+200mg/kg oral). Group III was administered ED (BV)+ED(ML)+colchicine (400mg/kg+200mg/kg+1mg/kg,i.c.v.). The fourth group got ED(BV)+ED(ML)+piracetam (400mg/kg+200mg/kg oral+200mg/kg, i.p.). Group V has been given low dose BV+low dose ML(200mg/kg +100 mg/kg oral) for 28 days and colchicine 1 mg/kg, i.p., at 60 min after 28th day piracetam injection. Group VI received high dose BV+high dose ML (400mg/kg + 200 mg/kg oral). M. longifolia and B. variegata ethanolic leaf extracts indicated an increase in transfer latency of mice in an elevated plus maze. No significant changes were observed in the locomotor activity in actophotometer. The transfer latency in the passive avoidance model showed a good increment. The M. longifolia and B. variegata leaf extract combination showed an increment (P<0.001) in SOD and catalase levels and a decrease (P<0.001) in MDA and total protein. The combination of M. longifolia and B. variegata has a neuroprotective effect against memory damage caused by colchicine.