Comprehensive virome profiling of sugarcane and simplified duplex OneStep RT-PCR assay reveals the prevalence of sugarcane streak mosaic virus along with sugarcane yellow leaf virus in India
The first comprehensive report of sugarcane virome and use of an optimized duplex OneStep RT-PCR assay revealed the prevalence of SCSMV and SCYLV in sugarcane from India. The study also provides an insight into genetic variations in the coding region of SCSMV and SCMV and emergence of diverse variants present in a viral population. A simplified duplex OneStep RT-PCR assay for simultaneous and expeditious detection of prevalent viruses in sugarcane would be useful in certification programme for production of virus-free planting materials.
Authors
- V. K. Baranwal (ORCID: https://orcid.org/0000-0002-5251-0905)
- Nishant Srivastava (ORCID: https://orcid.org/0000-0002-0443-1241)
- Vanita Chandel (ORCID: https://orcid.org/0000-0003-1300-1988)
- Susheel Kumar Sharma (ORCID: https://orcid.org/0000-0002-9114-4231)
- Malyaj R. Prajapati (ORCID: https://orcid.org/0000-0002-8542-9732)
- Nitika Gupta (ORCID: https://orcid.org/0000-0002-9087-1891)
- Rakesh Kumar (ORCID: https://orcid.org/0000-0002-4538-2554)
- Pooja Bhardwaj (ORCID: https://orcid.org/0009-0009-9093-7713)
Institutions
- Amity University (IN)
- Indian Agricultural Research Institute (IN)
Publication Details
- Journal
- Journal of Genetic Engineering and Biotechnology
- Published
- 2024-11-26
- DOI
- https://doi.org/10.1016/j.jgeb.2024.100442
- Citations
- 5
- Primary Topic
- Plant Virus Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 3.00
Funders
- National Research Centre
- Department of Biotechnology, Ministry of Science and Technology, India
- Indian Council of Agricultural Research
- Indian Agricultural Research Institute