Genomic surveillance of multidrug-resistant organisms based on long-read sequencing
We demonstrate that molecular characterization of automatically extracted DNA followed by long-read sequencing is as accurate compared to short-read sequencing and suitable for typing and outbreak analysis as part of genomic surveillance of MDRO. However, the analysis of P. aeruginosa requires further improvement which may be obtained by other basecalling algorithms. The low implementation costs and rapid library preparation for long-read sequencing of MDRO extends its applicability to resource-constrained settings and low-income countries worldwide.
Authors
- Wei Ang (ORCID: https://orcid.org/0000-0003-0849-4749)
- Eva Kolwijck
- S. Dinant
- A. J. van Griethuysen
- Angela de Haan (ORCID: https://orcid.org/0000-0003-1916-1835)
- E. van der Vorm
- Hanneke Berkhout
- Jerome R. Lo Ten Foe
- M. van Rijn (ORCID: https://orcid.org/0000-0002-0007-2448)
- Fabian Landman (ORCID: https://orcid.org/0000-0001-6543-836X)
- M. J. C. A. van Trijp
- Erik Bathoorn (ORCID: https://orcid.org/0000-0003-2319-8147)
- Sandra Witteveen (ORCID: https://orcid.org/0000-0001-9132-9028)
- Nashwan al Naiemi (ORCID: https://orcid.org/0000-0002-0176-9853)
- S Vainio
- T. Schülin (ORCID: https://orcid.org/0000-0002-0441-1654)
- Daan W. Notermans (ORCID: https://orcid.org/0000-0002-9494-0989)
- L. Bode (ORCID: https://orcid.org/0000-0002-2096-1015)
- A. G. M. Buiting
- Rosa van Mansfeld
- S. Paltansing
- Leo M. Schouls (ORCID: https://orcid.org/0000-0003-1253-9794)
- B. Zwart
- M den Reijer
- J. de Vries (ORCID: https://orcid.org/0000-0003-2533-3808)
- A. L. M. Vlek
- Alje P. van Dam (ORCID: https://orcid.org/0000-0003-3967-550X)
- Anouk E. Muller (ORCID: https://orcid.org/0000-0001-5042-6251)
- Radjin Steingrover
- J. C. Sinnige
- Ianthe Maat
- E. Heikens
- Karin van Dijk (ORCID: https://orcid.org/0000-0002-2054-2857)
- Wouter van den Bijllaardt (ORCID: https://orcid.org/0000-0003-0676-2594)
- Andreas van Arkel (ORCID: https://orcid.org/0000-0003-3391-9200)
- Marcus Ho Yin Wong (ORCID: https://orcid.org/0000-0001-5472-8337)
- Annet Troelstra
- Damian C. Melles∗
- Alewijn Ott
- Antoni P. A. Hendrickx (ORCID: https://orcid.org/0000-0003-4431-9323)
- Casper Jamin (ORCID: https://orcid.org/0000-0003-0244-0467)
- B. M. W. Diederen
- B. Maraha (ORCID: https://orcid.org/0000-0003-2639-9024)
- E.M.G.J. de Jong (ORCID: https://orcid.org/0000-0003-3872-5704)
- P Gruteke (ORCID: https://orcid.org/0000-0003-1788-5960)
- J. da Silva
- Martin de Graaf (ORCID: https://orcid.org/0000-0001-7948-3292)
- J W Dorigo-Zetsma
- K. Waar
- Jeroen Bos (ORCID: https://orcid.org/0009-0001-3278-2591)
- M. A. Hall
- A Jansz
- D. W. van Dam
- J. W. T. Cohen Stuart
- Han G. J. van der Heide
- S. P. van Mens
- E. I. G. B. de Brauwer
- Maurits P. A. van Meer
- M. P. M. van der Linden
- S. B. Debast
Institutions
- National Institute for Public Health and the Environment (NL)
Publication Details
- Journal
- Genome Medicine
- Published
- 2024-11-25
- DOI
- https://doi.org/10.1186/s13073-024-01412-6
- Citations
- 29
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 4.94
Funders
- Oxford Nanopore Technologies
- Ministerie van Volksgezondheid, Welzijn en Sport
- Rijksinstituut voor Volksgezondheid en Milieu