Genomic Insights into Stutzerimonas kunmingensis TFRC-KFRI-1 Isolated from Manila Clam (Ruditapes philippinarum): Functional and Phylogenetic Analysis
<i>Stutzerimonas kunmingensis</i> TFRC-KFRI-1, isolated from the gut of Manila Clam in the sediment of the West Sea of Korea, was investigated for its potential as a probiotic bacterium. This strain, belonging to the family <i>Pseudomonadaceae</i>, was previously classified as <i>Pseudomonas kunmingensis</i> but later reclassified to the genus <i>Stutzerimonas</i>, known for species with bioremediation and probiotic properties. To evaluate its genomic features and potential applications, we performed draft-genome sequencing and analysis. The genome of <i>S. kunmingensis</i> TFRC-KFRI-1 was assembled into a 4,756,396 bp sequence with a 62.8% GC content. Genomic analysis suggested potential genes for carbohydrate degradation and lactic acid production. The strain exhibited high average nucleotide identity (ANI) and 16S rRNA similarity with <i>S. kunmingensis</i> HL22-2<sup>T</sup>, further supporting its potential as a probiotic. This genome sequence provides valuable insights into the functional capabilities of <i>S. kunmingensis</i> TFRC-KFRI-1 and its potential applications in various industries, including aquaculture and food biotechnology. The genome sequence is available under GenBank accession number JBGJJB000000000.1, with related project information under BioProject PRJNA1147901 and Bio-Sample SAMN43173893.
Authors
- Jiho Choi (ORCID: https://orcid.org/0000-0001-8839-4056)
- Myunglip Lee (ORCID: https://orcid.org/0009-0006-5139-0733)
- Sung-Hun Yi (ORCID: https://orcid.org/0000-0003-0540-5771)
- Yucheol Kim (ORCID: https://orcid.org/0000-0001-8197-586X)
- Chaehyeon Lim (ORCID: https://orcid.org/0000-0002-9977-561X)
- Y. Eugene Pak (ORCID: https://orcid.org/0000-0002-4013-9192)
Institutions
- Korea Food Research Institute (KR)
- National Institute of Fisheries Science (KR)
Publication Details
- Journal
- Microorganisms
- Published
- 2024-11-23
- DOI
- https://doi.org/10.3390/microorganisms12122402
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Ministry of Oceans and Fisheries
- National Institute of Fisheries Science